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１９８９年Ｂｒｉｓｓｏｎ－Ｎｏｅｌ首次成功地把多聚酶链式反应（ＰＣＲ）技术应用于结核病原学诊断。国内、外实验室室间质控研究表明，该方法检测实验室标本的正确率可达到１００％，但实际临床应用中还存在许多问题尚待解决，特别是痰标本的留取和处理中的干扰因素较多
In 1989 Brisson-Noel successfully applied polymerase chain reaction (PCR) to the diagnosis of tuberculosis. Laboratory studies at home and abroad show that the accuracy of this method for detecting laboratory specimens can reach 100%. However, there are still many problems to be solved in practical clinical applications, especially in the collection and treatment of sputum specimens Interference factors more