人工锌指核酸酶的设计与应用

来源 :畜牧与兽医 | 被引量 : 0次 | 上传用户：gloria_yan

【摘要】

：

锌指核酸酶是一种人工合成的融合蛋白,由特异性识别靶DNA的锌指蛋白结构域连接非特异性的FokI切割结构域构造而成,能够在预定的DNA靶位点引起DNA双链中断,随后依靠非同源末端

【作者】

：

邓廷贤庞春英杨炳壮梁贤威

【机构】

：

中国农业科学院广西水牛研究所

【出处】

：

畜牧与兽医

【发表日期】

：

2004年期

【关键词】

：

锌指蛋白锌指核酸酶基因修饰基因治疗

【基金项目】

：

科技部国际科技交流与合作专项;

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锌指核酸酶是一种人工合成的融合蛋白,由特异性识别靶DNA的锌指蛋白结构域连接非特异性的FokI切割结构域构造而成,能够在预定的DNA靶位点引起DNA双链中断,随后依靠非同源末端连接和同源重组修复两种途径修饰该位点.近些年来,该技术已广泛的应用于基因转变、基因修饰和基因治疗等方面的研究.本文将简要介绍锌指核酸酶结构与原理,重点对锌指核酸酶的设计和应用进行了回顾与展望.

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人工锌指核酸酶的设计与应用

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