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目的探讨肺炎支原体肺炎(MPP)患儿外周血中IL-35与巨噬细胞、树突状细胞水平的相关性。方法采用酶联免疫吸附试验(ELISA)法检测治疗前MPP患儿和健康对照者外周血IL-35的含量,分析MPP患儿外周血中IL-35的变化。测定MPP患儿和健康对照组外周血中的巨噬细胞、树突状细胞的表达水平,并探讨两者与IL-35的相关性。结果患儿组血清IL-35水平高于对照组,差异有统计学意义(P<0.01)。患儿组全血中巨噬细胞含量高于对照组,差异有统计学意义(P<0.01)。患儿组全血中的树突状细胞含量低于对照组,差异有统计学意义(P<0.05)。IL-35含量的变化与树突状细胞百分含量的变化呈负相关(r=-0.722,P<0.01);与巨噬细胞百分含量的变化呈正相关(r=0.539,P<0.01)。结论通过控制体内IL-35含量来增加或减少体内相应的树突状细胞、巨噬细胞含量,来控制抗原的递呈过程,进而控制免疫反应的强弱,可能为MPP的免疫治疗提供理论依据。
Objective To investigate the correlation between IL-35 and macrophages and dendritic cells in children with Mycoplasma pneumoniae pneumonia (MPP). Methods The content of IL-35 in peripheral blood of MPP children and healthy controls before and after treatment were detected by enzyme linked immunosorbent assay (ELISA), and the changes of IL-35 in peripheral blood of children with MPP were analyzed. The levels of macrophages and dendritic cells in peripheral blood of children with MPP and healthy controls were determined, and their correlation with IL-35 was also explored. Results The level of serum IL-35 in children was higher than that in control group (P <0.01). The content of macrophages in the whole blood of children group was higher than that of the control group, the difference was statistically significant (P <0.01). The content of dendritic cells in the whole blood of children group was lower than the control group, the difference was statistically significant (P <0.05). The change of IL-35 content was negatively correlated with the percentage of dendritic cell (r = -0.722, P <0.01), and positively correlated with the percentage of macrophage (r = 0.539, P <0.01) . Conclusion It is possible to provide a theoretical basis for the immunotherapy of MPP by controlling the level of IL-35 in the body to increase or decrease the corresponding dendritic cells and macrophages in vivo, so as to control the antigen presentation process and thus the immune response .