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目的:建立高效液相色谱法(HPLC)法测定清火胶囊中芦荟大黄素、大黄素和大黄酚含量的方法。方法:采用Eclipse XDB(4.6mm×250mm,5μm)色谱柱;流动相为甲醇-0.1%磷酸溶液(80∶20),流速为0.80mL/min,检测波长:254nm,柱温30℃。结果:芦荟大黄素、大黄素和大黄酚与其相邻质峰能完全分离。芦荟大黄素、大黄素和大黄酚的线性范围分别为:0.63~31.5μg/mL(r=0.999 9)、0.34~17.2μg/mL(r=1.000 0)、0.72~36.0μg/mL(r=0.999 8)。方法回收率均不低于97%。结论:本方法简便、准确、重现性好,能排除其他成分的干扰,可用于该制剂的质量控制的评价。
Objective: To establish a method for the determination of aloe-emodin, emodin and chrysophanol in Qinghuai capsule by high performance liquid chromatography (HPLC). Methods: The column was Eclipse XDB (4.6mm × 250mm, 5μm). The mobile phase was methanol-0.1% phosphoric acid solution (80:20), the flow rate was 0.80mL / min, the detection wavelength was 254nm and the column temperature was 30 ℃. Results: Aloe-emodin, emodin and chrysophanol can be completely separated from the adjacent mass peaks. The linear ranges of aloe-emodin, emodin and chrysophanol were 0.63 ~ 31.5μg / mL (r = 0.999 9), 0.34 ~ 17.2μg / mL (r = 1.000 0), 0.72 ~ 36.0μg / mL 0.999 8). Method recovery rate of not less than 97%. Conclusion: The method is simple, accurate, reproducible and can eliminate the interference of other components and can be used for the quality control evaluation of the preparation.