目的建立辣椒中苏丹红染料的液质联用检测方法。方法样品由正己烷提取,Sep Pak Alumina N固相萃取柱净化,使用Waters AcquityTMBEH C18色谱柱分离,以乙腈-0.1%甲酸水溶液作为流动相,经串联四级杆质谱多反应监测模式检测。结果此方法的检测限达到0.01μg/kg,3个不同添加水平的回收率为80%~97%。结论该方法灵敏、准确,可用于辣椒中苏丹红染料的测定。 Objective To establish a liquid chromatography-mass spectrometric method for the detection of Sudan dyes in pepper. Methods Samples were extracted with n-hexane, cleaned with a Sep Pak Alumina N solid phase cartridge and separated on a Waters AcquityTMBEH C18 column using acetonitrile-0.1% formic acid in water as the mobile phase for detection by a tandem quadrupole mass spectrometry multiple reaction monitor. Results The detection limit of this method was 0.01 μg / kg, and the recoveries of three different addition levels were 80% -97%. Conclusion The method is sensitive and accurate and can be used for the determination of Sudan dyes in pepper.