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目的:建立一种新型的集乙醇,四氯化碳(CCl4),刀豆蛋白A(Con A)三种刺激因素的复合式肝损伤小鼠模型,用于肝损伤及其治疗药物的研究。方法:取健康小鼠100只,雌雄各50只,随机分为五组,其中A组为空白对照组;B至E组为药物造模组。除空白对照组外,其余各小组连续四周复合式造模给药。于第4周末采血,分别测定谷草转氨酶(AST)、谷丙转氨酶(ALT)活性,取肝脏用于切片观察。结果:模型B组(乙醇+四氯化碳)和模型E组(乙醇+四氯化碳+刀豆蛋白A)小鼠血清中AST、ALT含量与空白对照组相比均有非常显著性差异(P 0.01),并且切片显示B组和E组动物肝细胞病变,肝脏损伤,与空白对照组相比有明显的差异。结论:用多因素刺激可建立较为理想的复合式肝损伤小鼠模型。
OBJECTIVE: To establish a new mouse model of hepatic injury induced by hepatic injury and its therapeutic agents with ethanol, carbon tetrachloride (CCl4) and concanavalin A (Con A). Methods: 100 healthy mice were randomly divided into five groups, 50 in each group, in which group A was blank control group and group B to E were drug model group. Except for the blank control group, the rest of the teams were given compound administration for four consecutive weeks. Blood samples were collected on the fourth weekend, and the activities of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were determined. The liver was taken for slicing. Results: The levels of AST and ALT in model group B (ethanol + carbon tetrachloride) and model group E (ethanol + carbon tetrachloride + concanavalin A) were significantly different from those of the blank control group (P <0.01), and the slices showed the liver cell lesions and liver injury in group B and group E compared with the blank control group. Conclusion: Multi-factor stimulation can establish an ideal composite model of liver injury in mice.