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目的观察二甲双胍对晚期糖基化终末产物(AGEs)诱导的大鼠颅骨成骨细胞增殖、分化、矿化的影响。方法分离培养大鼠颅骨成骨细胞,四氮唑蓝(MTT)比色分析法测定细胞增殖,生化法测定碱性磷酸酶(ALP)活性、茜素红S钙染法检测矿化结节形成,盐酸脱钙法检测矿化结节中钙含量。结果 500μg/ml AGEs抑制成骨细胞增殖、ALP活性、钙化结节形成、钙沉积;给予二甲双胍(100～500μmol/L)可不同程度上提高成骨细胞数量和ALP活性,促进矿化结节形成及钙沉积,减轻AGEs对成骨细胞增殖、ALP活性、钙化结节形成及钙沉积的抑制。结论 AGEs对原代成骨细胞增殖、分化与矿化产生抑制作用,二甲双胍提高成骨细胞的成骨能力,减轻AGEs对成骨细胞功能的损害。
Objective To observe the effects of metformin on the proliferation, differentiation and mineralization of rat calvarial osteoblasts induced by advanced glycation end products (AGEs). Methods Rat calvarial osteoblasts were isolated and cultured. Cell proliferation was measured by MTT colorimetric assay. Alkaline phosphatase (ALP) activity was measured by biochemical method. Alizarin red S calcium staining was used to detect mineralized nodules , Hydrochloric acid decalcification method to detect mineralized nodules in calcium content. Results 500μg / ml AGEs could inhibit osteoblast proliferation, ALP activity, calcification nodule formation and calcium deposition. Metformin (100-500μmol / L) could increase osteoblast number and ALP activity to some extent and promote mineralized nodule formation And calcium deposition, reduce AGEs on osteoblast proliferation, ALP activity, calcification nodules and calcium deposition inhibition. Conclusion AGEs can inhibit the proliferation, differentiation and mineralization of primary osteoblasts. Metformin can enhance the osteogenic capacity of osteoblasts and reduce the damage of AGEs to the function of osteoblasts.