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本研究用体外培养人胚肺二倍体成纤维细胞作为壮龄剂抗老化研究的细胞模型,从35代开始,于培养液中加入壮龄剂者为实验组,不加药为对照组。现察了壮龄剂对二倍体细胞的形态结构、生长速度和增殖的影响。结果是实验组细胞的传代寿命为68代,对照组为51代,实验组比对照组多传17代,能延长细胞寿命33.3％代。实验组细胞的生长状态,速度和酸性磷酸酶活性变化均比同代对照组细胞好。这些结果证明了壮龄剂有促进细胞生长增殖和抗老化作用。
In this study, human embryonic lung diploid fibroblasts were cultured in vitro as a cell model for the study of antiaging effects of ageing agents. From the 35th generation onwards, the addition of the agent to the growth medium was used as the experimental group, and no drug was added as the control group. The effects of ageing agents on the morphological structure, growth rate and proliferation of diploid cells were examined. As a result, the passage lifetime of the cells in the experimental group was 68 generations and the control group was 51 generations. The experimental group passed 17 generations more than the control group, prolonging the cell lifespan by 33.3%. The growth state, speed and acid phosphatase activity of the experimental group were better than those of the control group. These results demonstrate that younger age agents promote cell proliferation and anti-aging effects.