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目的:探讨不同剂量脉络宁注射液对SD大鼠脑缺血-再灌注损伤的治疗作用,并探索最佳给药剂量。方法:将45只SD大鼠随机(随机数字法)分为假手术组、缺血-再灌注(I-R)组、低剂量组(20g·Kg-1·d-1)、中剂量组(40g·Kg-1·d-1)和高剂量组(80g·Kg-1·d-1)。每组各9只。采用Longa法制作脑缺血-再灌注模型。造模成功后6h进行神经功能评分。低、中、高剂量组分别给予相应剂量脉络宁注射液,每天一次,连用7d。注射体积为1.5ml。假手术组和缺血-再灌注组给予相应体积的等渗盐水。所有大鼠14d后评分、取材。流式细胞仪检测大鼠海马凋亡细胞,电镜观测海马CA1区神经元细胞超微结构。结果:各治疗组神经功能评分与缺血-再灌注组比较差异有显著统计学意义(P<0.01);低剂量组与中、高剂量组比较有统计学差异(P<0.05);各治疗组凋亡细胞数与缺血-再灌注组比较有较明显统计学差异(P<0.01);低剂量组与中、高剂量组比较亦有较明显差异(P<0.01)。治疗组大鼠海马CA1区神经元超微结构较缺血-再灌注组明显改善;而中、高剂量组海马CA1区神经元超微结构较低剂量组有明显改善。结论:脉络宁注射液对脑缺血-再灌注损伤具有治疗作用,并通过抑制神经细胞凋亡、促进神经细胞恢复改善预后。且以中等(40g·Kg-·1d-1)以上剂量效果最好。
Objective: To investigate the therapeutic effect of different doses of Mailuoning injection on cerebral ischemia-reperfusion injury in SD rats and explore the optimal dosage. Methods: Forty-five SD rats were randomly divided into sham operation group, IR group, low dose group (20 g · Kg-1 · d-1) and middle dose group (40 g · Kg-1 · d-1) and high-dose group (80g · Kg-1 · d-1). Each group of 9. Longa method was used to make cerebral ischemia - reperfusion model. 6h after modeling success neurological score. Low, medium and high dose groups were given the corresponding dose Mailuoning injection, once daily, once every 7d. The injection volume is 1.5 ml. Sham operation group and ischemia-reperfusion group were given the corresponding volume of isotonic saline. After 14 days, all rats were scored and drawn. The apoptotic cells were detected by flow cytometry in hippocampus of rats. The ultrastructure of hippocampal CA1 neurons was observed by electron microscope. Results: There was significant difference between the neurological function score and the ischemia-reperfusion group in each treatment group (P <0.01), the difference between the low dose group and the medium and high dose groups was statistically significant (P <0.05) The number of apoptotic cells was significantly different from that of ischemia-reperfusion group (P <0.01). There was also significant difference between low dose group and middle dose group and high dose group (P <0.01). The ultrastructure of hippocampal CA1 neurons in the treatment group was significantly improved compared with the ischemia-reperfusion group, while the neurons in the hippocampal CA1 region of the middle-dose and high-dose groups were significantly improved. Conclusion: Mailuoning injection has a therapeutic effect on cerebral ischemia - reperfusion injury and improves the prognosis by inhibiting the apoptosis of nerve cells and promoting the recovery of nerve cells. And medium (40g · Kg- · 1d-1) more than the best dose.