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目的:探讨抑制过氧化物酶1(Prx-1)对肝癌细胞放射敏感性的影响。方法:选用人肝癌Hep G2细胞,用分次放疗放射剂量递增法诱导放射抵抗的肝癌细胞(RR-Hep G2),检测RR-Hep G2细胞与亲代Hep G2细胞中Prx-1的表达,以及两种细胞在接受相同放射处理后存活率与迁移、侵袭能力。用表达sh RNA-Prx-1的质粒转染两种细胞后,再次检测上述指标的变化。结果:与亲代Hep G2细胞比较,RR-Hep G2细胞Prx-1的m RNA与蛋白表达明显增高;放射处理后,RR-Hep G2细胞的存活率升高,迁移能力及侵袭能力增强(均P<0.05)。转染sh RNA-Prx-1后,与各自转染阴性对照序列的细胞比较,两种细胞Prx-1的m RNA与蛋白表达均明显降低;接受放射处理后,两种细胞的存活率均降低,迁移能力以及侵袭能力均明显减弱(均P<0.05)。结论:肝癌细胞对放射的敏感性降低可能与Prx-1的表达有关,调控Prx-1的表达可望成为增强肝癌细胞放射敏感性的有效途径。
Objective: To investigate the effect of inhibiting Prx-1 on the radiosensitivity of hepatocellular carcinoma cells. Methods: Human hepatocellular carcinoma Hep G2 cells were selected. Radioimmunoassay (RR-Hep G2) was induced by radioimmunotherapy. The expression of Prx-1 in RR-Hep G2 cells and Hep G2 cells was detected. Seed cells in the same radiation treatment after survival and migration, invasion ability. After transfection of both cells with the plasmid expressing sh RNA-Prx-1, the changes of the above indexes were examined again. Results: The mRNA and protein expression of Prx-1 in RR-Hep G2 cells were significantly increased compared with that of parental Hep G2 cells. After radiation treatment, the survival rate of RR-Hep G2 cells was increased, and the migration ability and invasive ability were enhanced <0.05). After transfection with sh RNA-Prx-1, the mRNA and protein expressions of Prx-1 in both cells were significantly decreased compared with those transfected with negative control sequences. After irradiation, the survival rates of both cells decreased , Migration ability and invasive ability were significantly weakened (all P <0.05). Conclusion: The decreased sensitivity of hepatoma cells to radiation may be related to the expression of Prx-1. Regulation of Prx-1 expression may be an effective way to enhance the radiosensitivity of hepatoma cells.