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目的:完善脑络通胶囊的质量标准,以更好控制产品质量。方法:用理化方法对脑络通胶囊中维生素B_6和TLC法对丹参、黄芪、甲基橙皮苷、川芎进行鉴别;采用HPLC法测定原儿茶醛、甲基橙皮苷及维生素B_6的含量。结果:理化方法能检出维生素B_6,用TLC法均能检出丹参、黄芪、甲基橙皮苷、川芎。原儿茶醛在0.08～0.49μg范围内线性关系良好,r= 0.9999,平均回收率为101.6%,RSD为1.7%(n=6);甲基橙皮苷在0.22～2.16μg范围内线性关系良好,r=0.9999,平均回收率为99.6%,RSD为1.6%(n=6);维生素B_6在0.04～0.4μg范围内线性关系良好,r=0.9999,平均回收率为99.9%,RSD为3.1%(n=6)。结论:方法操作简单,可以用来控制脑络通胶囊的质量。
Objective: To improve the quality standard of Naoluotong Capsules to better control product quality. Methods: Physiological and chemical methods were used to determine the contents of protocatechuic aldehyde, methyl hesperidin and vitamin B 6 in Boli Luo capsule and TLC method of Salvia miltiorrhiza, Astragalus, methyl hesperidin and Chuanxiong rhizome. . Results: Physical and chemical methods can detect vitamin B_6, TLC method can detect Salvia, Astragalus, methyl hesperidin, Chuanxiong. Protocatechuic aldehyde showed good linearity in the range of 0.08 ~ 0.49μg with r = 0.9999, the average recovery was 101.6% and the RSD was 1.7% (n = 6). There was a linear relationship between methyl hesperidin and 0.22 ~ 2.16μg The average recovery was 99.6% and the RSD was 1.6% (n = 6). The calibration curve of vitamin B 6 was linear in the range of 0.04-0.4 μg (r = 0.9999) with the average recovery of 99.9% and the RSD of 3.1 % (n = 6). Conclusion: The method is simple and can be used to control the quality of Naoluotong capsule.