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目的探讨促性腺激素释放激素拮抗剂和激动剂抗原主动免疫对小鼠免疫效果、生长与器官发育和生殖内分泌的作用。方法 105只昆明雌鼠随机均分为西曲瑞克组(CET)、曲普瑞林组(TRI)和对照组(CG),在CET-1、CET-2及CET-3小组分别皮下注射曲普瑞林抗原0.1、0.2及0.4 ml,TRI-1、TRI-2及TRI-3小组分别皮下注射西曲瑞克抗原0.1、0.2及0.4 ml,CG组注射生理盐水0.2 ml,各组均连续注射7 d。精确测定体质量,于0、21及35 d从各小组分别抓取5只小鼠眼眶后静脉丛采血,分离血清;同时断颈处死,无菌采集心脏、肝脏、脾脏、肾脏、子宫及卵巢,分别称质量,计算日增质量和内脏指数。ELISA试剂盒测定血清Gn RH抗体、LH和Gn RH质量浓度。结果实验期间CET-3组体质量大于CG组(P<0.01),CET-1和CET-2组小鼠体质量在12 d和21 d时小于CG组(P<0.05);8 d后TRI-1小组体质量低于CG组(P<0.05);TRI-2和TRI-3组分别于16 d和21 d降至低谷。CET-3和TRI-1组肝脏与脾脏质量均明显大于CG(P<0.05),35 d时CET-3、TRI-1、TRI-2和TRI-3组子宫质量分别减小31.63%、21.55%、63.1%和61.71%;CET-1和CET-2组子宫质量比CG增加40.78%和11.94%。CET和TRI的血清Gn RH抗体浓度高于CG,且TRI组的高于CET组,尤其是TRI-3组更明显(P<0.01)。21 d各实验组Gn RH质量浓度均低于CG,TRI-3组显著低于CG(P<0.05)。至35 d,CET-1、CET-2、TRI-2和TRI-3组Gn RH质量浓度显著低于CG(P<0.05),CET-3组则高于CG。CET-3和TRI-3组血清LH质量浓度显著低于CG。结论西曲瑞克和曲普瑞林主动免疫能剂量依赖性地促进机体产生Gn RH抗体,免疫抑制Gn RH和LH合成与分泌,低剂量免疫注射初期抑制小鼠生长,高剂量后期明显促进肝脏和脾脏的生长发育、抑制子宫发育,且曲普瑞林的作用更加明显。
Objective To investigate the effects of gonadotropin-releasing hormone (HGH) antagonist and active autoantibody against immune function, growth, organ development and reproductive endocrine in mice. Methods 105 female Kunming mice were randomly divided into CET, TRI group and control group (CG), and were subcutaneously injected with CET-1, CET-2 and CET-3 respectively The 0.1, 0.2 and 0.4 ml triptorelin antigens were injected subcutaneously in the TRI-1, TRI-2 and TRI-3 groups respectively. Continuous injection of 7 d. The body weight was accurately measured. Five orbital venous plexuses were harvested from each group on 0, 21 and 35 days for blood collection and serum separation. The heart, liver, spleen, kidney, uterus and ovary , Respectively, said the quality, calculated daily gain and visceral index. Serum Gn RH antibody, LH and Gn RH mass concentrations were measured by ELISA kit. Results The body mass of CET-3 group was significantly higher than that of CG group during the experiment (P <0.01). The body weight of CET-1 and CET-2 group was smaller than CG group at 12 and 21 d (P <0.05) -1 group was lower than that of the CG group (P <0.05). The TRI-2 and TRI-3 groups decreased to the lowest level on the 16th and 21st days respectively. The liver and spleen mass of CET-3 and TRI-1 groups were significantly higher than that of CG group (P <0.05). On the 35th day, the mass of the uterus in CET-3, TRI-1, TRI-2 and TRI-3 groups decreased by 31.63% and 21.55 %, 63.1% and 61.71% respectively. The uterine mass of CET-1 and CET-2 groups increased by 40.78% and 11.94%, respectively. Serum Gn RH antibody concentrations of CET and TRI were higher than that of CG, and TRI group was higher than CET group, especially TRI-3 group (P <0.01). The concentrations of Gn RH in experimental groups were all lower than that of CG on 21 d, and were significantly lower in CGI than that of CG in TRI-3 group (P <0.05). At 35 days, the concentrations of Gn RH in CET-1, CET-2, TRI-2 and TRI-3 groups were significantly lower than those in CG (P <0.05). Serum LH concentrations in CET-3 and TRI-3 groups were significantly lower than those in CG. Conclusion Active immunization with cetrorelix and triptorelin can promote Gn RH antibody production, Gn RH and LH synthesis and secretion in a dose-dependent manner, inhibit the growth of mice at the early stage of low dose immunization, And spleen growth and development, inhibition of uterine development, and the role of triptorelin more pronounced.