论文部分内容阅读
目的:探讨原发性高血压内皮一氧化氮合酶基因eNOSG894T变异与高胰岛素血症的关系及其临床意义。方法:非糖尿病原发性高血压患者150例,根据空腹胰岛素水平分为非高胰岛素血症、高胰岛素血症及明显高胰岛素血症;正常血压对照组70例。运用聚合酶链反应限制性片段长度多态性检测各组eNOSG894T变异,比较各组eNOSG894T变异基因携带或非携带者的代谢指标。结果:明显高胰岛素血症患者eNOSG894T等位基因频率显著高于正常血压对照组及非高胰岛素血症者(分别为24·6%,10·7%,13·7%,均P<0·01);明显高胰岛素血症患者eNOSG894T变异基因携带者的收缩压、平均动脉压、餐后血糖、三酰甘油及尿酸明显高于正常血压对照组,高密度脂蛋白则明显低于正常血压对照组(均P<0·05)。结论:eNOSG894T变异基因与高胰岛素血症密切相关,可作为高血压患者高危预后评估的指标。
Objective: To investigate the relationship between eNOSG894T polymorphism of endothelial nitric oxide synthase gene and hyperinsulinemia in patients with essential hypertension and its clinical significance. Methods: 150 cases of non-diabetic patients with essential hypertension were divided into non-hyperinsulinemia, hyperinsulinemia and hyperinsulinemia according to the level of fasting insulin; 70 cases of normal blood pressure control group. The eNOSG894T mutation in each group was detected by polymerase chain reaction restriction fragment length polymorphism. The metabolic indexes of eNOSG894T variant gene in each group were compared with those in non-carriers. Results: The frequency of eNOSG894T allele in patients with hyperinsulinemia was significantly higher than those in normotensive subjects and non-hyperinsulinemic patients (24.6%, 107.7%, 13.7%, respectively, P <0 · 01). The systolic blood pressure, mean arterial pressure, postprandial blood glucose, triglyceride and uric acid in eNOSG894T variant gene carriers were significantly higher in patients with hyperinsulinemia than in those in normal blood pressure control group, and those in HDN patients were significantly lower than those in normotensive subjects Group (all P <0.05). CONCLUSION: The eNOSG894T variant gene is closely related to hyperinsulinemia and can be used as an indicator of high-risk prognosis in hypertensive patients.