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目的研究不同浓度葡萄糖对巨噬细胞高密度脂蛋白受体(SR-BI)及凝集素样氧化型低密度脂蛋白受体(LOX-1)表达的影响。方法2003年5月至2005年1月在中国医科大学附属第一医院将5.6mmol/L、11.1mmol/L、16.7mmol/L和33.3mmol/L葡萄糖与诱导分化48h后的U937细胞共同孵育24h及16.7mmol/L葡萄糖与诱导分化48h后的U937细胞作用0,12,24,48,72h后,用Western印迹法检测SR-BI及LOX-1蛋白的表达。结果葡萄糖对巨噬细胞SR-BI蛋白表达量无显著影响(P>0.05)。葡萄糖浓度升高及作用时间延长可使LOX-1蛋白表达增加。结论葡萄糖诱导LOX-1蛋白表达增加并呈浓度和时间依赖性。
Objective To investigate the effects of different concentrations of glucose on the expression of high density lipoprotein receptor (SR-BI) and lectin-like oxidized low density lipoprotein receptor (LOX-1) in macrophages. Methods From May 2003 to January 2005, 5.6 mmol / L, 11.1 mmol / L, 16.7 mmol / L and 33.3 mmol / L glucose were respectively incubated with U937 cells induced for 48 h after differentiation for 48 h at the First Affiliated Hospital of China Medical University And 16.7mmol / L glucose for 48h. The expression of SR-BI and LOX-1 protein were detected by Western blotting at 0,12,24,48 and 72h. Results Glu had no significant effect on the expression of SR-BI in macrophages (P> 0.05). LOX-1 protein expression increased with the increase of glucose concentration and the prolongation of action time. Conclusion Glucose induces LOX-1 protein expression in a concentration-and time-dependent manner.