OBJECTIVE To investigate the apoptosis-inducing effect ofXIAP antisense oligonucleotides on glioblastoma cells in vitro.METHODS There were 4 groups in our experiment. Group A,as a cell control group, had normal cell culture and no treatmentapplied. Group B, as a blank control group, had normal cellculture and no liposome control of ASODN. Group C was N-ODN.Group D was the ASODN group. RT-PCR and Western blot assaywere conducted to detect the expression of XIAP in all A-172cell groups after treatment with XIAP antisense oligonucleotides(ASODN). MTT assay and flow-cytometry (FCM) detection wereused to detect the ability of cell anchoring growth and apoptoticrates of all groups. The processing time was 72 h.RESULTS The expression of XIAP in the A-172 cells was greatlydown-regulated, after treated with XIAP-ASODN. Amongdifferent concentrations of ASODN, the 300nM was the mostoptimal one. The down-regulation of XIAP obviously inhibited thesuccinate dehydrogenase (SDH) activity of the A-172 cells and theincreased apoptotic rate of A-172 cells (87.45%) was significantlyhigher than that of the A-172 in the control groups. There wasa statistically significant difference between the treatment andcontrol groups (P < 0.01).CONCLUSION The XIAP-ASODN can effectively regulate theexpression of the XIAP down, as a result, inhibit the growth of theglioblastoma cells (A-172) and obviously increase the apoptoticrate of the A-172 cells. The results of the study manifest an overtkilling role of XIAP-ASODN to the glioblastoma cells. OBJECTIVE To investigate the apoptosis-inducing effect of XIAP antisense oligonucleotides on glioblastoma cells in vitro. METHODS There were 4 groups in our experiment. Group A, as a cell control group, had normal cell culture and no treatment applied. Group B, as a blank control group, had normal cell culture and no liposome control of ASODN. Group C was N- ODN. Group D was the ASODN group. RT-PCR and Western blot assay were conducted to detect the expression of XIAP in all A-172 cell groups after treatment with XIAP Antisense oligonucleotides (ASODN). MTT assay and flow-cytometry (FCM) detection were used to detect the ability of cell anchoring growth and apoptoticrates of all groups. The processing time was 72 h.RESULTS The expression of XIAP in the A-172 cells was greatly down-regulated, after treated with XIAP-ASODN. Among different concentrations of ASODN, the 300 nM was the most optive one. The down-regulation of XIAP are inhibited the succinate dehydrogenase (SDH) activity of the A-172 There was a significant difference between the treatment and control groups (P <0.01). CONCLUSION The XIAP-ASODN can effectively regulate the expression of the XIAP down, as a result, inhibit the growth of the glioblastoma cells (A-172) and obviously increase the apoptotic rate of the A-172 cells. The results of the study manifest an overtilling role of XIAP-ASODN to the glioblastoma cells.