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以川梨Pyrus pashia Buch.-Ham.、豆梨P.calleryana Decne.和杜梨P.betulaefolia Bunge果实以及嫁接在这3种砧木上的沙梨品种“丰水”果实为试材,采用高效液相色谱,比较了3种砧木果实与接穗品种果实中有机酸组分和含量变化特点,探讨了砧木对接穗品种果实中有机酸代谢的影响。结果表明,供试砧木和接穗品种果实中有机酸种类上并无显著性差异,均含有草酸、奎宁酸、苹果酸、莽草酸和柠檬酸等5种有机酸,不同砧木对接穗品种丰水梨果实中有机酸代谢具有调控作用,杜梨可以降低丰水梨果实中柠檬酸的含量,导致苹果酸含量与柠檬酸含量的比值升高,从而提高果实品质,梨果实中苹果酸和柠檬酸的含量变化与砧木种类有关,特别是当果实中苹果酸和柠檬酸的含量无显著性差异时,将梨果实有机酸代谢类型分为苹果酸优势型和柠檬酸优势型的分类方法难以应用。
Using the fruits of Pyrus pashia Buch.-Ham., P.calleryana Decne. And P.betulaefolia Bunge, and the pear cultivars “Fengshui” which were grafted on the three kinds of rootstocks, Liquid chromatography was used to compare the changes of organic acids and their contents in fruit of three kinds of rootstocks and scion varieties. The effects of rootstocks on organic acids metabolism in the fruit of scion were also discussed. The results showed that there was no significant difference in the organic acids in the fruits of tested rootstocks and scion varieties, including oxalic acid, quinic acid, malic acid, shikimic acid and citric acid. Five kinds of organic acids such as scion, Fruit organic acid metabolism has a regulatory role, Du pear can reduce citric acid content in pear fruit, resulting in malic acid content and citric acid content ratio increased, thereby enhancing the fruit quality, pear fruit malic acid and citric acid content The changes are related to the types of rootstocks. Especially when there is no significant difference in the content of malic acid and citric acid in the fruit, it is difficult to apply the classification of organic acid metabolism types of pear fruit to the dominant type of malic acid and citric acid.