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目的探讨Th17、Th22及Treg细胞频数及相关细胞因子变化在儿童川崎病(KD)发病机制中的作用。方法收集本院自2013年收治的KD初诊患者42例(KD初诊组),缓解患者42例(KD缓解组),以同期健康体检者30例作为正常对照组(HC组),以流式细胞仪检测3组外周血Th17、Th22细胞及Treg细胞频数,以酶标仪检测血浆中细胞因子IL-17、IL-10、IL-22及IL-6。结果 KD初诊组Th17、Th22细胞比例较HC组、KD缓解组均明显升高,差异均有统计学意义(P<0.05);Treg细胞比例较HC组、KD缓解组均明显下降,差异有统计学意义(P<0.05);KD初诊组细胞因子IL-17、IL-22、IL-10、IL-6较KD缓解组及HC组均明显升高,差异有统计学意义(P<0.05)。结论 Th22、Th17细胞和Treg细胞及相关细胞因子变化可能参与KD的发病机制,临床上可以通过检测Th17、Th22、Treg细胞及相关细胞因子变化评估儿童KD的病情进展。
Objective To investigate the role of Th17, Th22 and Treg cell frequency and related cytokines in the pathogenesis of childhood Kawasaki disease (KD). Methods Forty-two patients with KD newly diagnosed (KD newly diagnosed) and 42 patients with complete remission (KD remission) were enrolled in our hospital from 2013, and 30 healthy controls were recruited as normal control group (HC group) IL-17, IL-10, IL-22 and IL-6 in plasma were detected by ELISA with Th17, Th22 and Treg cells in peripheral blood. Results The proportion of Th17 and Th22 cells in newly diagnosed KD group was significantly higher than that in HC group and KD remission group (P <0.05), and the percentage of Treg cells was significantly lower than that in HC group and KD remission group (P <0.05). The levels of cytokines IL-17, IL-22, IL-10 and IL-6 in KD newly diagnosed group were significantly higher than those in KD remission group and HC group . Conclusions The changes of Th22, Th17 cells and Treg cells and related cytokines may be involved in the pathogenesis of KD. The progression of KD in children may be assessed clinically by detecting the changes of Th17, Th22, Treg cells and related cytokines.