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目的:建立莪红胶囊的质量标准。方法:采用TLC法对莪红胶囊中红花、葛根、制何首乌进行定性鉴别;采用双波长HPLC法对莪红胶囊中羟基红花黄色素A、葛根素进行含量测定,色谱柱为Wondasil C_(18)-WR(250 mm×4.6 mm,5μm),流动相为甲醇-乙腈-0.7%磷酸水溶液(16∶3∶81),流速为1.0 ml·min~(-1),检测波长为403 nm(测羟基红花黄色素A)、250 nm(测葛根素),柱温为30℃。结果:TLC斑点清晰,分离度良好,阴性对照无干扰;羟基红花黄色素A、葛根素分别在各自进样量范围内与峰面积呈良好线性关系,相关系数均在0.999 9以上,平均加样回收率分别为101.02%、100.03%,RSD分别为1.43%、2.40%(n=6)。结论:该方法快速、准确、专属性强,可作为莪红胶囊质量控制的方法。
Objective: To establish the quality standard of red curcumin. Methods: The contents of safflower yellow, Pueraria lobata and Polygonum multiflorum were identified by TLC. The content of hydroxysafflor yellow A and puerarin in Curcuma longaensis was determined by dual-wavelength HPLC. The chromatographic column was Wondasil C_ 18) -WR (250 mm × 4.6 mm, 5 μm) with a mobile phase of methanol-acetonitrile-0.7% phosphoric acid (16:3:81) at a flow rate of 1.0 ml · min -1 and a detection wavelength of 403 nm (Measured hydroxysafflor yellow A), 250 nm (puerarin), the column temperature was 30 ℃. Results: The TLC spots were clear and the resolution was good. There was no interference with the negative control. The concentrations of hydroxysafflor yellow A and puerarin were linearly correlated with the peak area in the respective injection volume, the correlation coefficients were above 0.999 9, The recoveries were 101.02% and 100.03%, respectively, with RSDs of 1.43% and 2.40%, respectively (n = 6). Conclusion: The method is rapid, accurate and specific and can be used as a method for quality control of Curcuma longa capsule.