在植物远缘杂交育种工作中,经常由于父本花粉不能在母本柱头上萌发,或者花粉管在花柱中生长缓慢,甚至于不能达到子房,最后精子不能和卵细胞结合,有时虽能受精,但合子不能正常发育,因而杂交不能获得成功。近年来采用的植物子房和胚珠的离体培养,植物的试管受精以及胚胎培养的方法,可以完全有效地排除柱头,花柱对于受精的障碍,避免子房脱落,并利用培养条件促进受精后胚的发育。这是克服远缘杂交不亲和性,获得新类型的有效方法。国外有人为了得到油菜和白菜杂交后代,他们通过一 In plant distant hybridization and breeding work, pollen often fails to germinate on the stigma of the female parent, or the pollen tube grows slowly in the style or even can not reach the ovary. In the end, the spermatozoa can not combine with the egg cell, sometimes fertilizing, But Zizi can not be normal development, so hybridization can not be successful. In recent years, plant ovary and ovule in vitro culture, plant test tube fertilization and embryo culture method can be completely and effectively ruled out stigma, style barrier for fertilization, to avoid ovary shedding, and the use of culture conditions to promote fertilized embryos Development. This is an effective way to overcome the incompatibility of distant crosses and gain new types. In order to get the cross between offspring of rapeseed and cabbage, some people abroad passed one