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细胞的ATP能反映细胞代谢,是最灵敏的粉尘致巨噬细胞的细胞毒性指标。本实验采用沸水抽提法提取ATP,实验结果表明:沸水抽提法简单、可靠,而且测得的ATP含量较常用的酸提法提高1～2倍;用沸水抽提法,通过荧光酶测定仪检测肺泡巨噬细胞中的ATP含量,能为尘肺研究提供新的手段。
Cell ATP can reflect cell metabolism, is the most sensitive dust-induced cytotoxicity of macrophages. In this experiment, ATP was extracted by boiling water extraction. The experimental results showed that the method of boiling water extraction was simple and reliable, and the ATP content was 1 ~ 2 times higher than that of the common acid extraction method. The detection of ATP in alveolar macrophages can provide a new method for the study of pneumoconiosis.