替莫唑胺联合二甲双胍对胶质瘤干细胞的清除作用及其机制

来源 :吉林大学学报(医学版) | 被引量 : 0次 | 上传用户:goooooooal
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
目的:探讨替莫唑胺(TMZ)联合二甲双胍(MET)对体外胶质瘤干细胞(GSCs)的清除作用,探讨其作用机制。方法:神经干细胞培养基培养人胶质瘤U87细胞,免疫荧光法鉴定GSCs。收集GSCs,以对照液、不同浓度TMZ、MET和TMZ+MET作用细胞,分为对照组、TMZ组、MET组和TMZ+MET组。显微镜下计数各组二次神经球的数量;CCK-8法检测各组GSCs增殖抑制率;流式细胞术和Annexin-PI双染流式细胞术检测GSCs各细胞周期百分比和凋亡率。结果:与对照组比较,TMZ+MET组二次神经球数量以浓度依赖的方式减少;与TMZ和MET组比较,TMZ+EMT组二次神经球数量明显减少(P<0.01)。与对照组比较,TMZ和MET组GSCs增殖抑制率升高;TMZ+MET组GSCs增殖抑制率高于TMZ或MET组,联合指数(CI)小于1。流式细胞术,与TMZ和MET组比较,TMZ+MET组G2/M期GSCs百分比明显升高(P<0.05);TMZ+MET组GSCs凋亡率明显高于TMZ和MET组(P<0.05)。结论:TMZ联合MET在体外能抑制GSCs的连续自我更新能力并清除GSCs,其机制可能与阻滞GSCs细胞周期进展和诱导细胞凋亡有关联。 Objective: To investigate the scavenging effect of temozolomide (TMZ) combined with metformin (MET) on glioma stem cells (GSCs) in vitro and its mechanism of action. Methods: Human glioma U87 cells were cultured in neural stem cell culture medium and GSCs were identified by immunofluorescence. The GSCs were collected and divided into control group, TMZ group, MET group and TMZ + MET group with control liquid, different concentrations of TMZ, MET and TMZ + MET. The number of secondary neurospheres in each group was counted under microscope. The proliferation inhibition rate of GSCs in each group was measured by CCK-8 method. The percentage of cell cycle and the percentage of apoptotic cells were detected by flow cytometry and Annexin-PI double staining flow cytometry. Results: Compared with control group, the number of secondary neurospheres in TMZ + MET group decreased in a dose-dependent manner. Compared with TMZ and MET groups, the number of secondary neurospheres in TMZ + EMT group decreased significantly (P <0.01). Compared with the control group, the proliferation inhibition rate of GSCs in TMZ and MET groups increased. The proliferation inhibition rate of TMZ + MET group was higher than TMZ or MET group, and the combined index (CI) was less than 1. Flow cytometry showed that the percentage of GSCs in G2 / M phase in TMZ + MET group was significantly higher than that in TMZ and MET groups (P <0.05), and the apoptotic rate of TMZ + MET group was significantly higher than that in TMZ and MET groups (P <0.05) ). CONCLUSION: TMZ combined with MET can inhibit the continuous self-renewal ability of GSCs and clear GSCs in vitro. The mechanism may be related to arresting cell cycle progression and inducing apoptosis of GSCs.
其他文献
回 回 产卜爹仇贱回——回 日E回。”。回祖 一回“。回干 肉果幻中 N_。NH lP7-ewwe--一”$ MN。W;- __._——————》 砧叫]们羽 制作:陈恬’#陈川个美食 Back to yield
女性网络参政已成为当前政治民主化进程中的重要议题。女性参政模式从较为单一的制度性分配,逐步发展成为以"自我赋权"为核心的多元途径。借助社交媒体,运用各种"另类"的表达
《粤港澳大湾区发展规划纲要》提出,要构建现代化的综合交通运输体系,提升珠三角港口群国际竞争力,建设世界级机场群,畅通对外综合运输通道,构筑大湾区快速交通网络,提升客货
聋儿早期干预主要由新生儿普遍早期筛查和早期康复组成,如何做好两项工作的衔接,是做好早期干预的关键.作者介绍了浙江省听力障碍儿童早期干预的工作机制、工作措施和工作成
由农业社会向工业社会过渡,即实现工业化,是人类社会走向富强的必由之路。1949年新中国成立后,中国共产党人完成了国家独立的历史任务。在此基础上,实现国家的繁荣富强又成为
针对电子商务交易风险的识别率低与评估难等问题,本文充分利用贝叶斯网络和层次分析法在进行风险的分析控制上的优势,构建了一种以贝叶斯网络和层次分析法为基础的电子商务交