本次建立了从续随子(Euphorbia lathyris)种子中快速分离巨大戟二萜醇(ingenol)的方法,ingenol中的一种酯类具有抗癌及抗HIV活性。本法可限制皮肤刺激性油产生,而且能分离出大环二萜类成分。实验所用的续随子种子购自德国。 分离方法:将续随子种子(2kg)碾碎,用丙酮浸提,24h后过滤,用TLC检识为指标检测滤液直至显示提取完全。回 This time a method for the rapid isolation of ingenol from the seed of Euphorbia lathyris was established. An ester in ingenol has anti-cancer and anti-HIV activity. This method can limit the production of skin irritating oils and can separate macrocyclic diterpenoids. The follower seeds used in the experiment were purchased from Germany. Separation method: The continuous seed (2kg) was crushed, extracted with acetone, and filtered after 24h. The filtrate was detected with TLC as an indicator until the filtrate was completely extracted. Return