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目的分析江西省麻疹病毒分离株的分子生物学特征,掌握江西省目前麻疹病毒流行株的基因特性。方法采用淋巴信号激活因子转染的非洲绿猴肾细胞(Vero/SLAM)对2008年-2012年疑似麻疹患者咽拭子标本进行麻疹病毒分离,分离到的毒株通过逆转录-聚合酶链反应(RT-PCR)方法扩增麻疹病毒血凝素(hemagglutinin,H)基因,并对扩增出的核苷酸序列进行测定,与Gen Bank中麻疹病毒各基因型参考株比较并分析毒株变异情况。结果 2008年-2012年江西省麻疹病毒分离株均为麻疹H1基因型,属于我国麻疹病毒的优势株。13株麻疹病毒分离株之间H基因氨基酸的同源性为98.1%~100.0%;与H1基因代表型China93-7的H基因氨基酸同源性为97.2%~98.1%;与中国疫苗株沪191株的H基因氨基酸同源性为94.8%~95.6%。结论 2008年-2012年江西省麻疹病毒流行株为H1基因型,说明江西省近年来麻疹的流行株仍然是H1基因型。
Objective To analyze the molecular biological characteristics of measles virus isolates in Jiangxi Province and to know the genetic characteristics of the measles virus epidemic strains in Jiangxi Province. Methods Viral / SLAM was used to isolate measles virus from the pharyngeal swab specimens from suspected measles patients from 2008 to 2012. The isolated strains were analyzed by reverse transcription-polymerase chain reaction (H) gene of measles virus was amplified by reverse transcription-polymerase chain reaction (RT-PCR). The amplified nucleotide sequence was determined and compared with the reference strains of genotypes of measles in Gen Bank Happening. Results The isolates of measles virus in Jiangxi Province from 2008 to 2012 were all measles H1 genotypes and belonged to the predominant strains of measles virus in China. The amino acid homology of H gene between isolates of 13 measles isolates was 98.1% -100.0%. The amino acid homology of H gene with that of China93-7 was 97.2% -98.1% The amino acid homology of the H gene of the strain ranged from 94.8% to 95.6%. Conclusion The H1N1 genotype of measles virus in Jiangxi Province from 2008 to 2012 indicates that the epidemic strain of measles in Jiangxi Province in recent years is still H1 genotype.