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以五指山野生灵芝1号、2号、3号、4号、5号和对照7号灵芝为材料,研究4种母种培养基配方和4种原种培养基配方对野生灵芝菌种菌丝生长速度和生长势的影响,并比较分析不同灵芝品种中灵芝蛋白、多糖、三萜的含量。结果显示,在4种母种培养基上,菌丝生长速度的差异极显著,生长速度最快和生长势最强的是培养基配方B(PDA+木屑),最差的是配方D(PSA);在4种原种培养基上,生长速度和生长势强弱的先后顺序为配方3>配方4>配方2>配方1;在5种野生灵芝中,灵芝多糖、蛋白质、三萜含量最高的分别是5号(0.732 mg·g~(-1))、2号(0.088 mg·g~(-1))、5号(1.193 mg·g~(-1)),3种成分综合含量最高的是5号。该试验为五指山野生灵芝的开发利用提供了参考依据。
The fungus Ganoderma lucidum No.1, No.2, No.3, No.4, No.5 and No.7 Ganoderma lucidum were used as materials to study the effects of four kinds of parent culture medium and four kinds of original culture medium on the growth of wild Ganoderma lucidum Speed and growth potential, and comparative analysis of Ganoderma lucidum different varieties of Ganoderma lucidum protein, polysaccharide, triterpene content. The results showed that there were significant differences in the mycelium growth rate among the four kinds of maternal medium, the fastest growth rate and the strongest growth potential were medium formula B (PDA + sawdust), the worst was formula D (PSA) ; The order of growth rate and growth potential on the four kinds of original culture medium is formula 3> formula 4> formula 2> formula 1; Among the five kinds of wild ganoderma lucidum, the content of polysaccharide, protein and triterpene is highest The results showed that the contents of three components (0.732 mg · g -1), No.2 (0.088 mg · g -1) and No.5 (1.193 mg · g -1) It’s number 5 The experiment provides a reference for the development and utilization of wild fungus in Wuzhishan.