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目的:观察Y-box结合蛋白-1(Y-box binding protein 1,YB-1)对体外培养的大鼠肾小球系膜细胞(MC)增生及分泌转化生长因子-β1(TGF-β1)的影响。方法:体外培养大鼠肾小球系膜细胞株(HBZY-1),构建YB-1真核表达载体,转染系膜细胞,应用四甲基偶氮唑蓝(MTT)法及细胞计数法检测大鼠MC过表达YB-1后的增生情况,双抗体夹心ELISA法检测细胞培养上清液中TGF-β1分泌,real-time PCR法测定细胞TGF-β1基因相对表达量。结果:YB-1过表达后MC增生和TGF-β1基因表达增加,同时MC分泌TGF-β1增加。结论:YB-1能促进MC增生及TGF-β1分泌增加。
OBJECTIVE: To observe the effects of Y-box binding protein 1 (YB-1) on the proliferation and secretion of transforming growth factor-β1 (TGF-β1) in cultured rat glomerular mesangial cells Impact. Methods: Rat mesangial cell line (HBZY-1) was cultured in vitro. The eukaryotic expression vector of YB-1 was constructed and transfected into mesangial cells. MTT and cell counting The proliferation of rat MC was detected by YB-1 overexpression. The secretion of TGF-β1 in cell culture supernatant was detected by double antibody sandwich ELISA. The relative expression of TGF-β1 gene was determined by real-time PCR. Results: After YB-1 overexpression, MC proliferation and TGF-β1 gene expression increased, while MC secretion of TGF-β1 increased. Conclusion: YB-1 can promote MC proliferation and increase TGF-β1 secretion.