目的研究新疆苦豆子中黄酮类化学成分。方法采用大孔吸附树脂、聚酰胺柱层析法、SephadexLH-20柱色谱法及重结晶等方法对苦豆子中黄酮类化学成分进行研究,根据化合物的理化性质和光谱数据鉴定其结构。结果从新疆苦豆子中分离得到3种化合物,化学结构用一维和二维核磁共振技术分别鉴定为3′,4′-二羟基异黄酮-7-O-β-D-吡喃葡萄糖苷(Ⅰ)、7,3′-二羟基二氢黄酮-4′-O-β-D-吡喃葡萄糖苷(Ⅱ)、芦丁(Ⅲ)。结论化合物Ⅰ、Ⅱ、Ⅲ为首次从新疆苦豆子中分离得到。 Objective To study the flavonoids in Sophora alopecuroides from Xinjiang. Methods The macroporous resin, polyamide column chromatography, Sephadex LH-20 column chromatography and recrystallization were used to study the chemical constituents of flavonoids in Sophora alopecuroides. The structures of the compounds were identified by the physicochemical properties and spectral data. Results Three compounds were isolated from Sophora alopecuroides, and their chemical structures were identified as 3 ’, 4’-dihydroxyisoflavone-7-O-β-D-glucopyranoside ), 7,3’-dihydroxyflavone-4’-O-β-D-glucopyranoside (Ⅱ) and rutin (Ⅲ). Conclusion Compounds Ⅰ, Ⅱ and Ⅲ were isolated from Xinjiang Sophora alopecuroids for the first time.