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目的在大鼠脑缺血再灌注损伤发生后,用自由基清除剂依达拉奉对其进行治疗,监测再灌注不同时间点脑组织Cas-pase-3蛋白表达情况。研究依达拉奉对脑缺血再灌注损伤的保护作用。方法制作短暂、局灶性脑缺血再灌注模型。分为正常组及假手术组(对照组)、脑缺血再灌注对照组(缺血组)、依达拉奉治疗组(治疗组),分别于缺血1h后进行再灌注。治疗组于再灌注后30min腹腔内及皮下各注射依达拉奉1次(按依达拉奉3mg/kg),30min后重复一次。设再灌注后2h、6h、12h、24h、48h不同时间点,各时间点以0.4%戊巴比妥钠深度麻醉后快速断头法将大鼠处死,以4%多聚甲醛灌注固定后取脑,行免疫组化检测各时间点脑组织Caspase-3表达阳性细胞数。结果缺血组再灌注后2h在大脑额叶和顶叶皮质和海马即可见到Caspase-3蛋白表达的阳性细胞,12h达高峰,24h后开始下降。治疗组各时间点Caspase-3表达水平较对照组明显降低(P<0.01)。结论依达拉奉可抑制Caspase-3的表达,对缺血再灌注损害的脑组织有明显的保护作用。
Objective To observe the expression of Cas-pase-3 protein at different time points after cerebral ischemia-reperfusion injury in rats with edaravone, a free radical scavenger. To study the protective effect of edaravone on cerebral ischemia-reperfusion injury. Methods A transient, focal cerebral ischemia-reperfusion model was made. The rats were divided into normal group and sham-operation group (control group), cerebral ischemia-reperfusion control group (ischemia group) and edaravone treatment group (treatment group). In the treatment group, edaravone was injected intraperitoneally and subcutaneously once every 30 minutes after reperfusion (3 mg / kg Edaravone), and the treatment group was repeated 30 minutes later. After 2h, 6h, 12h, 24h and 48h reperfusion, rats were sacrificed by deep decapitation with 0.4% sodium pentobarbital anesthesia at different time points after reperfusion, and fixed with 4% paraformaldehyde Brain and immunohistochemistry were used to detect the number of Caspase-3 positive cells in each time point. Results The positive cells expressing Caspase-3 protein in the frontal lobe, the parietal cortex and the hippocampus were observed 2h after reperfusion in ischemic group, reaching the peak at 12h and then decreasing after 24h. The expression of Caspase-3 at each time point in the treatment group was significantly lower than that in the control group (P <0.01). Conclusion Edaravone can inhibit the expression of Caspase-3 and protect the brain from ischemia-reperfusion injury.