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目的通过比较原发性肝细胞癌及其癌旁组织的转录差异,探讨血管紧张素转化酶(ACE)对原发性肝细胞癌发生的影响。方法采用荧光定量PCR法对13例原发性肝细胞癌及癌旁组织和3例正常肝组织中ACE1、ACE2及其受体AT1R和MAS的基因转录水平进行检测。结果 ACE1、AT1R在原发性肝细胞癌中基因转录水平较癌旁组织高,MAS基因转录水平在原发性肝细胞癌中基因转录水平较癌旁组织低,差异均有统计学意义(均P<0.01)。结论 ACE1、ACE2及其受体AT1R和MAS基因在原发性肝细胞癌发生中可能起调控作用。
Objective To investigate the effect of angiotensin converting enzyme (ACE) on the occurrence of primary hepatocellular carcinoma (HCC) by comparing the transcriptional differences between primary hepatocellular carcinoma and its adjacent tissues. Methods The gene transcription levels of ACE1, ACE2 and their receptors AT1R and MAS in 13 cases of primary hepatocellular carcinoma and adjacent normal tissues and 3 cases of normal liver tissues were detected by fluorescence quantitative PCR. Results The gene transcription level of ACE1 and AT1R in primary hepatocellular carcinoma was higher than that in paracancer tissues. The transcription level of MAS gene in primary hepatocellular carcinoma was lower than that in paracancerous tissues, both of which were statistically significant P <0.01). Conclusion ACE1, ACE2 and their receptors AT1R and MAS genes may play a regulatory role in the development of primary hepatocellular carcinoma.