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目的研究枸杞多糖(LBP)诱导人食管癌细胞Eca-109的凋亡作用及其作用机制。方法体外培养的人食管癌细胞Eca-109,细胞经不同剂量LBP处理后,采用MTT法测定细胞的增殖情况,流式细胞仪分析LBP对Eca-109细胞周期和凋亡的影响,用RT-PCR技术检测Eca-109细胞Survivin mRNA的表达。结果 LBP可明显抑制人食管癌细胞Eca-109的生长,并能诱导Eca-109细胞凋亡,凋亡率(%)分别为6.20±0.62、12.80±0.47和18.10±0.70,与对照组(0.78±0.91)%比较,差异有统计学意义(P<0.01);各剂量药物处理组的肿瘤细胞SurVivin mRNA表达水平均明显降低(P<0.01)。结论 LBP可抑制Eca-109细胞增殖,诱导细胞凋亡,该作用可能与下调细胞Survivin表达有关。
Objective To study the apoptosis of human esophageal cancer cell line Eca-109 induced by LBP and its mechanism. Methods The human esophageal cancer cell line Eca-109 cultured in vitro was treated with different doses of LBP. The cell proliferation was measured by MTT assay. The effect of LBP on the cell cycle and apoptosis of Eca-109 cells was analyzed by flow cytometry. Survivin mRNA expression in Eca-109 cells was detected by PCR. Results LBP could significantly inhibit the growth of human esophageal cancer cell line Eca-109 and induce the apoptosis of Eca-109 cells with the apoptotic rates (%) of 6.20 ± 0.62, 12.80 ± 0.47 and 18.10 ± 0.70, respectively. Compared with the control group ± 0.91)%, the difference was statistically significant (P <0.01). Survivin mRNA expression in tumor cells in each dose group was significantly decreased (P <0.01). Conclusion LBP can inhibit Eca-109 cell proliferation and induce apoptosis, which may be related to the down-regulation of Survivin expression.