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The purpose of this study is to isolate the active fraction from the crude extract of Prinsepia utilis Royle and to determine its antibacterial activity against the standard and drug-resistant bacteria strains in vitro. The Prinsepia utilis Royle was extracted in three portions by using petroleum ether, ethanol and water by the continuation recirculate extraction. The antibacterial activity of the extracts were evaluated by the determination of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) values. The results showed that the ethanol extract and aqueous extract of petroleum ether showed the weakest inhibition. MIC values of the aqueous Prinsepia utilis Royle were found that they had significant effects against bacteria, while ethanol extracts against three standard bacteria (S. aureus ATCC25923, E. coli 44102, Salmonella 50041) were 25,25, 50 mg·mL-1 and 50,100,100 mg·mL-1, MIC values of three drug-resistant bacteria (S. aureus GL17, E. coli EYAC08-56, Salmonella STQD2G.) were 50, 50, 50 mg·mL-1 and 100,100,200 mg·mL-1, respectively. MBC values of two extracts against three standard strains were 25,50,100 mg·mL-1 and 50,100, 200 mg·mL-1, MBC values of three drug-resistant bacteria were 100,100,100 mg·mL-1 and 200,200,200 mg·L-1, respectively.