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目的:探讨结直肠癌裸鼠转移模型组织中血管内皮生长因子(VEGF),内皮抑素(ES),血管生成素(ANG),血管抑素(AS),基质金属蛋白酶-9(MMP-9),组织金属蛋白酶抑制物(TIMP)变化及健脾消癌方干预的机制。方法:在BALB/c-nu裸鼠盲肠内注射HCT116细胞50μL,造裸鼠结直肠癌转移模型。将模型裸鼠分为模型组、健脾消癌方组(40 g·kg-1),另设空白组。所有动物灌胃给予相应药物4周后,蛋白免疫印迹法(Western blot)测定裸鼠结肠、肝、肺及盲肠肿瘤组织中相关血管因子蛋白表达。结果:HCT116结直肠癌模型裸鼠的结肠、肝、肺与肿瘤组织的VEGF,MMP-9,TIMP明显升高(P<0.05,<0.01);ANG在结肠、肝与肿瘤组织也明显升高(P<0.05,P<0.01);ES在结肠、肺与肿瘤组织显著降低,AS在肝、肺及肿瘤组织显著降低(P<0.05)。健脾消癌方组的结肠MMP-9,肿瘤TIMP,肝组织ANG,MMP-9及肺组织VEGF明显降低,肺组织AS明显升高(P<0.05)。结论:HCT116结直肠癌模型裸鼠VEGF,ANG,MMP-9在结肠、肝、肺及肿瘤组织中蛋白表达升高,ES,AS降低,TIMP升高;健脾消癌方能比较全面地改善机体抗肿瘤血管生成的能力。
Objective: To investigate the expression of vascular endothelial growth factor (VEGF), endostatin (ES), angiopoietin (ANG), angiostatin (AS), and matrix metalloproteinase-9 ), Tissue inhibitor of metalloproteinase (TIMP) changes and the mechanism of Jianpixiaohua intervention. Methods: Fifty microliters HCT116 cells were injected into the cecal of BALB / c-nu nude mice to establish the model of colorectal cancer metastasis in nude mice. The model nude mice were divided into model group, Jianpi Xiaocaofang group (40 g · kg-1), another blank group. All animals were given gavage for 4 weeks. Western blot was used to detect the expression of related vascular factors in colon, liver, lung and cecum tumor tissues of nude mice. Results: The expressions of VEGF, MMP-9 and TIMP in colon, liver, lung and tumor of HCT116 colorectal cancer model group were significantly increased (P <0.05, <0.01); ANG was also significantly increased in colon, liver and tumor tissues (P <0.05, P <0.01). The levels of ES in colon, lung and tumor were significantly decreased while the levels of AS in liver, lung and tumor were significantly decreased (P <0.05). The expressions of MMP-9, TIMP, ANG, MMP-9, and VEGF in the spleen and kidney cancer decoction group were significantly decreased (P <0.05). Conclusion: The protein expression of VEGF, ANG, MMP-9 in colorectal, hepatic, lung and tumor tissues of HCT116 colorectal cancer model increased, while ES, AS decreased and TIMP increased. Anti-tumor angiogenesis of the body’s ability.