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基质金属蛋白酶 - 9(MMP- 9)主要降解基底膜中的 和 型胶原 ,在细胞穿经基底膜的过程中起关键的作用。为探讨前单核细胞 THP- 1分化过程中 MMP- 9的表达及其信号传导途径 ,作者采用细胞粘附实验、流式细胞术和凋亡检测的方法 ,检测了佛波脂 (PMA)诱导 THP- 1分化过程中 ,PKC、PI3K、NF- κB抑制剂 CalphostinC、L Y2 940 0 2、PDTC和地塞米松对细胞粘附、MMP- 9表达及细胞凋亡的影响。结果表明 :在 THP- 1分化过程中有MMP- 9的表达 ,各种抑制剂及地塞米松可抑制细胞粘附及 MMP- 9的表达 ,Annexin V凋亡检测发现 PDTC是以诱导细胞凋亡的方式而发挥作用的。因此 ,MMP- 9可作为前单核细胞向单核细胞分化的标志 ,PKC、PI3K、NF- κB都参与了 MMP- 9上调的信号传导。抑制 MMP- 9的表达将有助于炎性细胞浸润性疾病的治疗。
Matrix metalloproteinase-9 (MMP-9), which mainly degrades collagen in the basement membrane, plays a key role in cell penetration through the basement membrane. To investigate the expression of MMP-9 and its signal transduction pathway during the differentiation of pro-monocytic THP-1 cells, we used the cell adhesion assay, flow cytometry and apoptosis assay to detect the induction of PMA Effect of PKC, PI3K, NF-κB inhibitor Calphostin C, LY2 940 0 2, PDTC and dexamethasone on cell adhesion, MMP-9 expression and apoptosis during THP-1 differentiation. The results showed that there was MMP-9 expression during THP-1 differentiation, and various inhibitors and dexamethasone could inhibit cell adhesion and MMP-9 expression. Annexin V apoptosis assay showed that PDTC induced cell apoptosis Way to play a role. Therefore, MMP-9 can be used as a marker of pre-monocyte differentiation into monocytes. PKC, PI3K and NF-κB are all involved in the up-regulation of MMP-9. Inhibiting the expression of MMP-9 contributes to the treatment of inflammatory cell infiltrates.