目的研究金莲花(Trollius chinensis Bunge)中的化学成分。方法采用大孔吸附树脂、正相硅胶、ODS、Sephadex LH-20等柱色谱法和制备型高效液相色谱法等分离技术对金莲花体积分数为60%的乙醇溶液提取物进行分离、纯化,并通过化合物的理化性质与波谱特征鉴定其结构。结果从体积分数为60%的乙醇溶液提取物中分离并鉴定了10个化合物,分别为cirsimarin(1)、icariside B6(2)、(+)-(R)-腈甲基-3-羟基氧吲哚[(+)-(R)-cyanomethyl-3-hydroxyoxindole,3]、黑麦草内酯(loliolide,4)、腺嘌呤(adenine,5)、3,4-二羟基苯甲醛(3,4-dihydroxybenzaldehyde,6)、金合欢素-7-O-β-D-葡萄糖苷(acacetin-7-O-β-D-glucoside,7)、2“-O-(3’’’,4’’’-二甲氧基苯甲酰基)牡荆苷[2”-O-(3’’’,4’’’-dimethoxybenzoyl)vitexin,8]、pinoresinol-β-D-glucopyranoside(9)、芹菜素-8-C-(2“-O-阿魏酰基)-β-D-葡萄糖苷[apigenin-8-C-(2”-O-feruloyl)-β-D-glucoside,10]。结论化合物1-6为首次从金莲花属植物中分离得到。 Objective To study the chemical constituents of Trollius chinensis Bunge. Methods The macroporous resin, normal phase silica gel, ODS, Sephadex LH-20 column chromatography and preparative high performance liquid chromatography (HPLC) were used to separate and purify the ethanol extract with the volume fraction of 60% The structure of the compound was identified by its physicochemical properties and spectral characteristics. Results Ten compounds were isolated and identified as cirsimarin (1), icariside B6 (2), (+) - (R) - nitrile methyl- 3-hydroxyoxindole, 3], loliolide (4), adenine (5), 3,4-dihydroxybenzaldehyde -hydroxybenzaldehyde, 6) acactin-7-O-β-D-glucoside (7) Dimethoxybenzoyl vitexin, 8], pinoresinol-β-D-glucopyranoside (9), 2-O- Apigenin-8-C- (2’-O-feruloyl) -β-D-glucoside, 10] . Conclusion Compounds 1-6 were isolated from the genus Nasturtium for the first time.