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目的探讨雌激素治疗对绝经妇女外周血T淋巴细胞的影响。方法分离外周血T淋巴细胞,采用(Annexin V-FITC/PI双染色)测定淋巴细胞凋亡率,提取外周血淋巴细胞总RNA,RT-PCR法测定T淋巴细胞抗凋亡基因bcl-2及T淋巴细胞促进凋亡基因bax含量。结果绝经组T淋巴细胞凋亡率高于生育年龄组(<0.01)。绝经+E组T淋巴细胞凋亡率低于绝经组(<0.01)。绝经组Bcl-2表达低于生育年龄组(<0.01)。绝经+E组Bcl-2表达高于绝经组(<0.01)。绝经组Bax表达高于生育年龄组(<0.01);而绝经+E组Bax表达低于绝经组(<0.01)。结论雌激素可以降低绝经妇女外周血T淋巴细胞的凋亡,主要机制可能是提高抗凋亡基因Bcl-2的表达和降低促凋亡基因Bax的表达水平。
Objective To investigate the effect of estrogen on peripheral blood T lymphocytes in postmenopausal women. Methods Peripheral blood T lymphocytes were isolated and the rate of lymphocyte apoptosis was determined by Annexin V-FITC / PI double staining. The total RNA of peripheral blood lymphocytes was extracted. The anti-apoptotic gene bcl-2, T lymphocyte promotes the expression of bax in apoptosis gene. Results The apoptosis rate of T lymphocytes in the menopause group was higher than that in the reproductive age group (<0.01). Menopausal + E group T lymphocyte apoptosis rate was lower than the menopause group (<0.01). The expression of Bcl-2 in the menopause group was lower than that in the reproductive age group (<0.01). The expression of Bcl-2 in menopausal + E group was higher than that in menopause group (<0.01). The expression of Bax in menopause group was higher than that in childbearing age group (<0.01), while that in menopausal + E group was lower than that in menopause group (<0.01). Conclusion Estrogen can reduce the apoptosis of T lymphocytes in peripheral blood of postmenopausal women. The main mechanism may be to increase the expression of anti-apoptotic gene Bcl-2 and decrease the expression of pro-apoptotic gene Bax.