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目的探索膜型基质金属蛋白酶－１（ｍｅｍｂｒａｎｅ－ｔｙｐｅ１ ｍａｔｒｉｘ ｍｅｔａｌｌｏｐｒｏｔｅｉｎａｓｅ， ＭＴ_１－ＭＭＰ）与肝细胞癌（ＨＣＣ）侵袭转移性的关系及以ＭＴ_１－ＭＭＰ来判断肝细胞癌侵袭转移性的可能性和方法。方法通过ＲＴ－ＰＣＲ对正常肝、 ＨＣＣ及其癌旁组织、癌栓组织，裸鼠人肝癌高、低转移模型中的ＭＴ_１－ＭＭＰ ｍＲＮＡ进行半定量研究，并与ＨＣＣ侵袭转移的病理指标进行统计分析。结果正常肝、ＨＣＣ及其癌旁组织、癌栓组织，裸鼠人肝癌高、低转移模型中均有ＭＴ_１－ＭＭＰ ｍＲＮＡ表达，表达值分别为 １．１２± ０．７３、 ３．５１± ．９７、 １．１９± ０．５７和４．４５±１．１１；在癌组织，癌栓组织，裸鼠人肝癌高转移模型中ＭＴ_１－ＭＭＰ ｍＲＮＡ水平增高与ＨＣＣ侵袭转移性有关；癌组织ＭＴ_１－ＭＭＰ ｍＲＮＡ水平高于其癌旁组织，是通过ＭＴ_１－ＭＭＰ判断ＨＣＣ侵袭转移性的重要指标。结论ＭＴ_１－ＭＭＰ与ＨＣＣ侵袭转移性有关；癌组织ＭＴ_１－ＭＭＰｍＲＮＡ水平是否高于癌旁组织可作为通过ＭＴ_１－ＭＭＰ判断ＨＣＣ侵袭转移性的一项指标。
Objective To explore the relationship between membrane-type 1 matrix metalloproteinase (MT_1-MMP) and the invasion and metastasis of hepatocellular carcinoma (HCC) and the possibility and method of using MT_1-MMP to judge the invasion and metastasis of hepatocellular carcinoma. . Methods RT-PCR was used to semiquantitatively study the expression of MT1-MMP mRNA in the normal liver, HCC and its paraneoplastic tissues, tumor thrombus, and human hepatoma high and low metastasis models in nude mice. The results were compared with those of HCC invasion and metastasis. analysis. Results The expression of MT_1-MMP mRNA was detected in normal liver, HCC and its paraneoplastic tissues, tumor thrombus, and human hepatoma high and low metastasis models in nude mice. The expression values were 1.12± 0.73 and 3.51±, respectively. 97, 1.19± 0.57 and 4.45±1.11; The increase of MT_1-MMP mRNA in cancer tissue and tumor metastasis model of human hepatocellular carcinoma in nude mice was associated with invasion and metastasis of HCC; cancer tissue MT_1 - The level of MMP mRNA is higher than that in adjacent tissues, which is an important indicator of the invasion and metastasis of HCC through MT_1-MMP. Conclusion MT_1-MMP is related to the invasion and metastasis of HCC; whether the tumor tissue MT_1-MMP mRNA level is higher than that of the adjacent tissue can be used as an index to judge the invasion and metastasis of HCC through MT_1-MMP.