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纯化的马尾松毛虫质型多角体病毒（ＤｐＣＰＶ）云南文山株的病毒粒子经ＳＤＳＰＡＧＥ分析，其结构蛋白有１２０ｋｄ、１１６ｋｄ、１１０ｋｄ、６６ｋｄ和３３ｋｄ５个组分，而它的多角体蛋白含３０ｋｄ和２８ｋｄ两个主要组分。以纯化的ＤｐＣＰＶ粒子为抗原制备了２Ｄ５、２Ｄ１０、３Ｄ４和６Ｂ３共４种单克隆抗体，并测定了它们的亚类。制得的单克隆抗体用于ＤｐＣＰＶ的ＥＬＩＳＡ检测。对ＤｐＣＰＶ在棉铃虫卵巢细胞系ＳＦＥＨＡ８２１２和ＳＦＥＨＡ８３１中增殖动态的检测结果表明，ＤｐＣＰＶ感染培养细胞具有释放病毒量小和呈现持续感染等特点。用Ｗｅｓｔｅｒｎ印迹法在ＳＦＥＨＡ８３１细胞感染ＤｐＣＰＶ后第１８ｈ检测到病毒抗原的合成。运用所建立的免疫学检测方法，对几批采自ＤｐＣＰＶ防治林区的幼虫样品进行分析，结果表明，该方法适用于对ＤｐＣＰＶ的防治效果及其自然流行进行长时期、大规模的监测。
Purified Dendrolimus punctatus type polyhedrosis virus (DpCPV) Yunnan Wenshan virions by SDS PAGE analysis, the structural proteins 120kd, 116kd, 110kd, 66kd and 33kd 5 components, and its polyhedrin protein containing 30kd And 28kd two main components. Four monoclonal antibodies against 2D5, 2D10, 3D4 and 6B3 were prepared using purified DpCPV particles as antigen, and their subclasses were determined. The prepared monoclonal antibody was used for ELISA detection of DpCPV. DpCPV in the cotton bollworm ovarian cell line SFE HA 8212 and SFE HA 831 proliferation test results show that DpCPV infection of cultured cells with a small release of the virus and showed persistent infection and so on. Western blotting was used to detect the viral antigen synthesis at 18h after SFEHA831 cells were infected with DpCPV. Using the established immunological detection method, the larvae samples collected from DpCPV-controlled forest areas were analyzed. The results showed that this method was suitable for long-term and large-scale monitoring of the control effect and natural epidemic of DpCPV.