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目的:采用薄膜分散法制备鳖甲肽-长循环脂质体,并检测其表征。以超滤离心-HPLC法及透析法分别考察包封率及药物释放度。方法:采用薄膜分散法制备鳖甲肽-脂质体,检测粒径,PDI并观察脂质体电镜下形态。建立超滤离心-HPLC法测定方法并检测脂质体的包封率以筛选最佳药脂比;采用透析法考察药物在不同时间点的释放度。结果:采用超滤离心-HPLC法能有效测定鳖甲肽-脂质体的包封率,在药脂比1∶5时包封率最高。并且,在4 h时药物释放度最高,最后释放度趋于平稳。结论:本研究制备的脂质体粒径均匀性及形态良好,超滤离心-HPLC法可高效精确测定脂质体包封率,而透析法考察药物释放度稳定可靠,从而达到质量评价的目的。
OBJECTIVE: To prepare turtle peptide - long circulating liposomes by thin film dispersion method and test its characterization. Ultrafiltration centrifugation-HPLC and dialysis were investigated entrapment efficiency and drug release. Methods: The phagemid peptide - liposomes were prepared by thin film dispersion method. The particle size and PDI were measured and the morphology under liposome electron microscopy was observed. The method of ultrafiltration centrifugation-HPLC was established and the entrapment efficiency of liposomes was tested to select the best lipid-lipid ratio. The drug release at different time points was investigated by dialysis method. Results: The entrapment efficiency of turtle peptide-liposomes was determined by ultrafiltration centrifugation-HPLC method. The entrapment efficiency was the highest when the drug-lipid ratio was 1: 5. Moreover, the drug release was the highest at 4 h, and the release finally tended to be stable. Conclusion: The liposomes prepared in this study have good uniformity of particle size and morphology. Ultrafiltration centrifugation-HPLC method can measure the entrapment efficiency of liposomes with high efficiency and accuracy, while the dialysis method is stable and reliable in drug release, so as to achieve the purpose of quality evaluation .