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目的:应用体外实验研究探讨TGF-β1对鼻咽癌的作用机制。方法:应用免疫组化、MTT法和流式细胞仪技术,检测外源性TGF-β1对鼻咽癌细胞株生长、分化及凋亡等方面的影响。结果:TGF-β1受体TβRⅠ和TβRⅡ在鼻咽低分化CNE-2癌细胞株中均有表达,进一步给予梯度浓度的外源性TGF-β1作用于鼻咽癌CNE-2细胞株,应用MTT法发现外源性TGF-β1与对照组相比,能显著抑制CNE-2癌细胞株的生长,且最小显效浓度为0.1ng/mL,再用流式细胞仪进行检测,发现外源性TGF-β1作用后出现G1期阻滞、S期和G2/M期比例下降,凋亡比例则随着时间的延长逐渐增高且与对照组比较差异均有统计学意义。结论:TGF-β1/Smad信号传导通路可以通过调控鼻咽癌细胞的生长、细胞周期和凋亡等活动,从而参与鼻咽癌的发生和发展。
Objective: To investigate the mechanism of TGF-β1 on nasopharyngeal carcinoma in vitro. Methods: The effects of exogenous TGF-β1 on the growth, differentiation and apoptosis of nasopharyngeal carcinoma cell lines were detected by immunohistochemistry, MTT and flow cytometry. Results: The TGF-β1 receptor TβRⅠand TβRⅡwere expressed in nasopharyngeal poorly differentiated CNE-2 cancer cell lines. The concentration of exogenous TGF-β1 was further increased in nasopharyngeal carcinoma CNE-2 cell line. MTT Law found that exogenous TGF-β1 compared with the control group, CNE-2 cancer cell lines can significantly inhibit the growth, and the minimum effective concentration of 0.1ng / mL, and then detected by flow cytometry and found that exogenous TGF After G1 phase, G1 phase arrest, S phase and G2 / M phase ratio decreased, the proportion of apoptosis increased gradually with time, and the difference was statistically significant compared with the control group. Conclusion: The TGF-β1 / Smad signaling pathway can participate in the occurrence and development of nasopharyngeal carcinoma by regulating the growth, cell cycle and apoptosis of nasopharyngeal carcinoma cells.