研究了Z31×Q31、H×Z3、A188×B73三种玉米 (ZeamaysL .)基因型的愈伤组织对除草剂Bialaphos的耐性 ,从而确定 5mg·L-1为适宜的选择压。用基因枪将Zm13_Barnase基因转化玉米Q31×Z3、Z31×Q31的胚性愈伤组织 ,经 6轮的除草剂筛选获得 32块抗性愈伤组织 ,再生得到 2 4个植株。经PCR检测 ,18个植株PCR反应呈阳性。I_KI染色和花粉离体萌发观察发现 ,5个植株的花粉为部分不育。Southernblot分析证明 ,这 5个植株中整合有Bar nase基因。 The callus resistance of Zea mays L. Z31 × Q31, H × Z3 and A188 × B73 genotypes to the herbicide Bialaphos was studied, and 5 mg · L-1 was selected as the suitable selection pressure. Zm13_Barnase gene was transformed into embryogenic callus of maize Q31 × Z3 and Z31 × Q31 by gene gun, 32 resistant callus were obtained after 6 rounds of herbicide screening, and 24 plants were regenerated. The PCR reaction of 18 plants was positive by PCR. I_KI staining and in vitro pollen germination observed that pollen of 5 plants was partially sterile. Southern blot analysis demonstrated that Bar nase genes were integrated into these five plants.