论文部分内容阅读
目的:探讨环维黄杨星D长期给药对大鼠肝脏产生毒性的研究。方法:大鼠连续灌胃给药4、8周,观察不同剂量(3mg/kg、6mg/kg、12mg/kg)环维黄杨星D对血液生化指标及肝脏病理的影响,并采用蛋白免疫印迹法检测对肝组织钠钾ATP酶表达的影响。结果:环维黄杨星D给药4周,大剂量组(12mg/kg)血清谷草转氨酶(43.45±5.26)、谷丙转氨酶(201.51±22.79)显著高于对照组(P<0.05)。给药8周,小剂量组(3mg/kg)谷丙转氨酶(182.14±21.61)、中剂量组(6mg/kg)谷草转氨酶(28.56±5.03)、谷丙转氨酶(188.79±23.26),大剂量组(12mg/kg)谷草转氨酶(35.14±5.66)、谷丙转氨酶(193.26±22.49)较对照组明显升高(P<0.05),各剂量组(3mg/kg、6mg/kg、12mg/kg)大鼠肝脏病理表现均有不同程度变性坏死,同时大剂量组(12mg/kg)的钠钾ATP酶蛋白表达量(0.35±0.093)和中剂量组(6mg/kg)(0.75±0.133),较对照组显著下降。结论:环维黄杨星D对肝脏毒性可能与抑制Na+-K+-ATPase有关。
Objective: To investigate the toxicity of long-term administration of cyclophosphamide D in rat liver. Methods: The rats were given gavage for 4 weeks and 8 weeks respectively. The effects of different doses of 3, 6 mg / kg and 12 mg / kg cyclovulinomycin D on blood biochemical parameters and liver pathology were observed. Western blotting Effect of Assay on the Expression of Sodium and Potassium ATPase in Liver Tissues. Results: The serum aspartate aminotransferase (43.45 ± 5.26) and alanine aminotransferase (201.51 ± 22.79) in the high dose group (12mg / kg) were significantly higher than those in the control group (P <0.05). After 8 weeks of administration, alanine aminotransferase (182.14 ± 21.61) in the low dose group (3mg / kg), aspartate aminotransferase (26.56 ± 5.03) and alanine aminotransferase (188.79 ± 23.26) in the middle dose group (3mg / kg, 6mg / kg, 12mg / kg) were significantly higher than those in the control group (P <0.05). The levels of aspartate aminotransferase (ALT) At the same time, the expression of sodium and potassium ATPase in high dose group (0.35 ± 0.093) and middle dose group (6mg / kg) (0.75 ± 0.133) were significantly higher than those in control group Group decreased significantly. Conclusion: The toxicity of cyclovirobuxine D to liver may be related to the inhibition of Na + -K + -ATPase.