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目的：探讨痰聚合酶链反应（ＰＣＲ）ＴＢ－ＤＮＡ、血清中结核分支杆菌胞壁糖脂免疫球蛋白 Ｃ（ＬＡＭＩｇＧ）、卡介苗 免疫球蛋白 Ｇ（ＰＰＤ ＩｇＧ）及结核特异循环免疫复合物（ＳＣＩＣ）检测与结核菌素（ＰＰＤ ０．１ｕ）皮试，联合检测共同阳性 对菌阴肺结核的诊断价值。方法：病材取自初治菌阴肺结核３１例，健康对照组５３例，非结核肺疾病３０例，初治 菌阴肺结核５４例。采用同步检测方法。血清免疫学检测用酶联免疫吸附试验（ＥＬＩＳＡ）观察各组共同阳性率的变 化，对结果进行分析评价。结果：健康对照组２、３、４、５联阳性率均为０，特异性为１００％；初治菌阳组２、３、４、５联同 时阳性率分别为６７．７％，５８．１％，３２．３％，０％；初治菌阴组２、３、４．５联共同阳性率分别为４５．９％，１８．９％，２．７％， ０％；非结核肺疾病组２、３、４、５联合同时阳性率分别为２１．７％，１３．０％，０％，０％。结论：五种方法联合对健康组２、 ３、４、５联同时阳性检测特异性可达１００％，初治菌阴组阳性检出率２联可达４５．９％，比任何单项方法检出率要高， 由于原五种方法联合检测特异性有所下降，因此该方法更适用于初治菌阴肺结核的诊断，值得推广。
Objective: To investigate the relationship between sputum polymerase chain reaction (PCR) TB-DNA, serum Mycobacterium tuberculosis LAMIgG, PPD IgG and SCIC ) Test and tuberculin (PPD 0.1u) skin test, joint test jointly positive diagnostic value of bacteria and yin pulmonary tuberculosis. Methods: The diseased material was taken from 31 newly diagnosed and negative tuberculosis tuberculosis, 53 healthy control group, 30 non-tuberculosis pulmonary disease and 54 primary fresh-med tuberculosis. Using synchronous detection method. Serum immunological tests using enzyme-linked immunosorbent assay (ELISA) observed changes in the common positive rate of each group, the results were analyzed and evaluated. Results: The positive rate of 2,3,4,5 in the healthy control group was 0 and the specificity was 100%. The positive rates of 2,3,4 and 5 in the untreated group were 67.7% and 58% respectively. 1%, 32.3% and 0%, respectively. The common positive rates of 2,3,4.5 in the initial treatment group were 45.9%, 18.9%, 2.7% and 0%, respectively; The simultaneous positive rates of 2,3,4,5 in the disease group were 21.7%, 13.0%, 0% and 0% respectively. Conclusions: The combination of the five methods can detect 100% of the positive cases simultaneously in 2, 3, 4 and 5 cases of the healthy group, and the positive detection rate of the initial treatment group is up to 45.9% in 2 cases, compared with any single method The rate is high, the combined detection of the original five methods decreased specificity, so the method is more suitable for the diagnosis of initial treatment of overcast pulmonary tuberculosis, it is worth promoting.