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目的探讨芝麻叶多酚在体外对糖代谢相关的α-葡萄糖苷酶活性的抑制作用及机理。方法以4-硝基酚-α-D-吡喃葡萄糖苷(PNPG)为底物,利用酶-抑制剂模型及酶抑制动力学分析,考察芝麻叶多酚对α-葡萄糖苷酶的抑制活性及抑制类型。色谱柱为Hypersil ODSC18(250 mm×4.6 mm,5 m),流动相为2%(v/v)乙酸溶液(A)和2%(v/v)乙酸甲醇溶液(B),梯度洗脱,流速1.0 mL/min,检测波长280 nm,进样量20 L,高效液相色谱法分析SLP的化学成分。结果 SLP能显著抑制α-葡萄糖苷酶活性,半数抑制量(IC50)为1.00 mg/mL,抑制类型为可逆性非竞争抑制,抑制常数Ki为14.93 mg/L。色谱分析结果:SLP中主要含没食子酸,绿原酸,芹菜素,咖啡酸,对-香豆酸,柚皮苷,木犀草素和山奈酚等8种酚类化合物。结论芝麻叶多酚对α-葡萄糖苷酶活有明显抑制作用,可作为潜在的预防糖尿病的膳食功能因子。
Objective To investigate the inhibitory effect and mechanism of sesame leaf polyphenol on α-glucosidase activity related to glucose metabolism in vitro. Methods 4-Nitrophenol-α-D-glucopyranoside (PNPG) was used as a substrate to study the inhibitory activity of sesame-leaf polyphenol on α-glucosidase by enzyme-inhibitor model and enzyme inhibition kinetics And inhibition type. The chromatographic column was Hypersil ODSC18 (250 mm × 4.6 mm, 5 m) with a mobile phase of 2% (v / v) acetic acid (A) and 2% (v / v) The flow rate was 1.0 mL / min, the detection wavelength was 280 nm, the injection volume was 20 L, and the chemical composition of SLP was analyzed by high performance liquid chromatography. Results SLP could significantly inhibit the activity of α-glucosidase with an IC50 of 1.00 mg / mL. The inhibition type was reversible and non-competitive inhibition with an inhibition constant Ki of 14.93 mg / L. Results of chromatographic analysis: SLP mainly contains 8 kinds of phenolic compounds such as gallic acid, chlorogenic acid, apigenin, caffeic acid, p-coumaric acid, naringin, luteolin and kaempferol. Conclusion The polyphenol of sesame leaves can significantly inhibit the activity of α-glucosidase and serve as a potential dietary factor for preventing diabetes.