现已发现非促性腺激素,特别是胰岛素、皮质醇和甲状腺激素影响人和动物粒层细胞的体外生长和黄素化。Channing等发现胰岛素、皮质醇和甲状腺素在培养的猪粒层细胞内与黄体生成素(LH)和促卵泡成熟激素(FSH)协同作用而增强了形态上的黄素化和孕激素的分泌。他们还发现,皮质醇可使接触FSH的培养液中猪粒层细胞LH/HCG受体增多。因此,甲状腺激素似在体外粒层细胞有直接作用。此作用可能是受体介导的。据作者了解尚未见粒层细胞内有三碘甲腺氨酸(T_3)受体存在的报道。本文目的即在于证实在猪粒层细胞内存在T_3结合位 Non-gonadotropins, in particular insulin, cortisol and thyroid hormones, have been found to affect in vitro growth and luteinization of human and animal layers of cells. Channing et al found that insulin, cortisol and thyroxine enhanced the morphological luteinization and progestin secretion by synergistic effect with luteinizing hormone (LH) and follicle stimulating hormone (FSH) in cultured porcine granulosa cells. They also found that cortisol increased LH / HCG receptors in porcine stratum corneum cells exposed to FSH. Therefore, thyroid hormones seem to have a direct effect on the granulosa cells in vitro. This effect may be receptor mediated. According to the authors, there is no report on the presence of triiodothyronine (T_3) receptors in granulosa cells. The purpose of this paper is to confirm the presence of T_3 binding sites in porcine granulosa cells