论文部分内容阅读
采用聚合酶链反应（ＰＣＲ）技术扩增肺炎支原体特异的１４４ｂｐＤＮＡ片段靶基因，检测３５４例临床疑为呼吸道感染、肺炎、支气管炎病人咽拭标本，检出阳性７４例，总阳性率为２０．９％，其中儿科患者１９１例，阳性３８例，阳性率为１９．６％；老年患者８６例，阳性２０例，阳性率２３．２％；内科患者７７例，阳性１６例，阳性率２０．７％。ＰＣＲ利用体外基因复制技术，仅用１ｈ即可将极微量的ＤＮＡ样品特异地放大几百万倍，直接检出肺炎支原体。本文检测结果提示，不能忽视老年人支原体肺炎的发生，应引起临床医生的注意。
The target gene of Mycoplasma pneumoniae-specific 144 bp DNA fragment was amplified by polymerase chain reaction (PCR) and detected in 354 cases of clinically suspected throat swabs in patients with respiratory tract infection, pneumonia and bronchitis. The positive rate was 74, and the total positive rate was 20. Among them, 191 cases were pediatric patients, 38 cases were positive, the positive rate was 19.6%; 86 cases were elderly, 20 cases were positive, the positive rate was 23.2%; 77 cases were internal medicine, 16 cases were positive, the positive rate was 20%. 7%. PCR using in vitro gene replication technology, only 1h can be a trace amount of DNA samples specifically magnified millions of times, direct detection of Mycoplasma pneumoniae. This test results suggest that the incidence of mycoplasma pneumonia in the elderly can not be ignored, should attract the attention of clinicians.