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以ＭＳ液体培养基培养２０ｄ左右至菌丝大量生长并紧密交织成团时病菌即可大量产生毒素，病菌是否产孢对产毒量大小无显著影响。毒素原液中仅非蛋白质部分具致病活性，经高温高压灭菌１５ｍｉｎ后活性仍不丧失，且有所加强。该毒素在冷冻条件或灭菌后于室温下可保存两个月以上而不失活。毒素粗提液中活性成分为一类极性较大的物质，易溶于水、甲醇、乙醇，可溶于正丁醇，微溶于乙酸乙酯，不溶于苯，可被活性炭吸附并为甲醇所洗脱。毒素粗提液的ｐＨ值为６０，渗透势为－１２２ｋＰａ，经试验确定毒素粗提液的毒性非溶液的酸度或渗透势所致，从而进一步确定了病菌人工培养滤液中存在具致病活性的毒素物质。
MS liquid culture medium for about 20d to mycelium mass growth and closely interwoven into the mass bacteria can produce a large number of toxins, bacteria whether sporulation on the size of the production of no significant impact. Only the non-protein part of the toxin stock solution has the pathogenic activity, and the activity is still not lost after being sterilized by autoclaving for 15 minutes, and has been strengthened. The toxin can be stored at room temperature for more than two months without deactivation after being frozen or sterilized. The crude extract of toxins active ingredients for a class of more polar substances, soluble in water, methanol, ethanol, soluble in n-butanol, slightly soluble in ethyl acetate, insoluble in benzene, can be activated carbon adsorption and Methanol eluted. The toxin crude extract had a pH of 6.0 and an osmotic potential of -122 kPa. The acidity or osmotic potential of the toxic non-solution of the toxin crude extract was determined experimentally to further confirm the presence of pathogenic bacteria in the artificial culture filtrate Active toxins.