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【目的】了解临床护士对《中国糖尿病药物注射技术指南》中胰岛素注射技巧的掌握情况及向患者告知情况，比较《糖尿病药物注射指南》培训前后的差别。【方法】随机选取本院各临床科室护士90例，于培训前、培训后采用调查问卷进行效果评定。【结果】培训前护士对于胰岛素注射部位选择和注射部位消毒掌握较好（操作正确率均在90％以上），但对于注射前患者的心理疏导、注射部位轮换、检查等方面掌握并不理想（操作正确率78．％～733．％），且在这些方面对患者的教育较差（告知率33．％～544．％）；培训后，上述方面有所改善（操作正确率300．％～878．％，告知率278．％～744．％， P ＜00．5）。【结论】糖尿病药物注射指南培训能较好地促进临床护士对胰岛素笔注射技能相关知识的理解和掌握，并促进临床护士对糖尿病患者进行正确的教育。“,”Objective]To investigate the understanding of injection technique and patient education in clinical nurses , and compare their understandings before and after Diabetes Drug Injection Guide training .[Methods]Randomly chosen 90 clinical nurses were enrolled in our study .A questionnaire concerning injection technique was made before and after train‐ing .The questionnaire was designed according to the Chinese guideline .[Results]Before the training ,clinical nurses had a good understanding of both the position of injection and disinfection procedures (accuracy rate above 90% ) .However ,the clinical nurses had poor knowledge about the psychological counseling ,injection routine ,examination et al (accuracy rate of 7 8.~73 3.% ) ,and the situation of patient education was poor (accuracy rate of 3 3.~54 4.% ) .After the training ,the knowledge of clinical nurses were improved (accuracy rate after training was 30 0.~87 8.% ,the accuracy rate before train‐ing was 27 8.~74 4.% ,P <0 0.5) .[Conclusion]The training improved the understanding and grasping of injection tech‐nique among clinical nurses ,and promoted the situation of patient education .