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:目的 :比较国产氨舒钠 (ASN)和进口优力新 (ULX)制剂 (均由氨苄西林 (AMP)与舒巴坦 (SBT)以 2 :1比例组成 )对细菌β -内酰胺酶的稳定性 ,并检测SBT对 β -内酰胺酶的抑制作用。方法 :用紫外分光光度计测定酶对抗生素的水解率 ,并计算SBT的抑酶率。结果 :8种细菌 β -内酰胺酶对ASN和ULX的水解率 (5 15 %～ 7 5 9% )明显低于单用AMP的平均相对水解率31 13 % (P <0 0 5 ) ,而与IMI和CTX相当。以AMP(0 1mmol/L)为底物 ,发现SBT(0 0 6～ 5 μg/ml)对 4种 β -内酰胺酶的平均抑制率与药物浓度间呈显著的直线相关性 (γ =0 9943)。结论 :SBT增强AMP抗菌活性的作用可能与之抑酶作用有关
Objective: To compare the effects of domestic ammonia sulindac (ASN) and imported ULX agents (both consisting of ampicillin (AMP) and sulbactam (SBT) at a 2: 1 ratio) on bacterial β - lactamase Stability, and detect the inhibitory effect of SBT on β - lactamase. Methods: The enzyme hydrolysis rate of antibiotics was measured by UV spectrophotometer, and the inhibition rate of SBT was calculated. Results: The rates of hydrolysis of ASN and ULX by 8 bacterial β - lactamases were significantly lower than those of AMP alone (31 13%, P <0 05) Equivalent to IMI and CTX. Using AMP (0 1 mmol / L) as substrate, we found a significant linear correlation between the average inhibitory rate of four β - lactamases and SBT (0 0 6 ~ 5 μg / ml) 9943). Conclusion: SBT enhances the antimicrobial activity of AMP, which may be related to the inhibition of AMP