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采用 EAG生测技术研究表明 ,甜菜夜蛾雄蛾触角上具有 4种性信息素组分 (Z9,E1 2 - 1 4∶Ac(A)、Z9- 1 4∶ OH (B)、Z9- 1 4∶ Ac(C)和 Z9,E1 2 - 1 4∶ OH(D) )的感受细胞。单一组分 A,二元组分 AB和 AD,三元组分 ABC、ABD和 ACD及四元组分 ABCD的 EAG反应显著较高 ,与 3头雌蛾性信息素腺体浸提液的反应强度相当。进一步对 AB二元混合物中两组分的最佳比例以及最佳比例的剂量反应进行了测定。当 AB间的比例为 5∶ 5时可产生最大的 EAG反应 ,其它比例 (9∶ 1、7∶ 3、3∶ 7、1∶ 9)间无明显差异。 AB(5∶ 5)混合物的使用剂量在 0 .0 4～ 4μg范围内 ,EAG值随剂量增加呈直线上升 ;使用剂量低于 0 .0 4μg或高于 40μg,EAG值不再有明显变化
The results of EAG bioassay showed that there were four sex pheromone components (Z9, E1 2 - 1: 4A (A), Z9-1: OH (B), Z9-1 4: Ac (C) and Z9, E1 2 - 1 4: OH (D)). The EAG responses of single component A, binary components AB and AD, ternary components ABC, ABD and ACD and quaternary component ABCD were significantly higher than those of the female sex pheromone glands The same intensity. The optimal ratio of the two components in the AB binary mixture and the optimal ratio of the dose response were determined. The largest EAG response was produced when the ratio between ABs was 5: 5, with no significant difference between the other ratios (9: 1, 7: 3, 3: 7, 1: 9) The dosage of AB (5: 5) mixture was in the range of 0.4 ~ 4μg, the EAG value increased linearly with the increase of dosage; the dose of EAG was no more than 0.4μg or more than 40μg