论文部分内容阅读
目的:探讨生长抑制基因表达的蛋白p33ING1和端粒酶逆转录酶催化亚单位(hTERT)在新疆维吾尔族妇女宫颈鳞癌石蜡组织中的表达及二者的相互关系。方法:应用免疫组化Envision法检测宫颈浸润性鳞癌52例、宫颈上皮内瘤变35例患者中p33ING1和hTERT的表达,并与正常宫颈13例进行对照。结果:①对照组,CINII、I级组,CINIII级组和SCC组中p33ING1阳性率分别为84.6%,90%,64%,19.2%,SCC组中表达明显下降,与前三组之间差异有统计学意义(p<0.05);②四组中hTERT阳性率分别为15.4%,70%,96%,100%,宫颈癌组织中表达明显增高,各组之间差异有统计学意义(p<0.05);③p33ING1和hTERT的表达呈负相关(p<0.05),但它们的表达均和癌组织的分化程度无显著差异。结论:新疆维吾尔族妇女宫颈癌组织中p33ING1表达明显下降,而hTERT表达明显增加,二者之间有一定的相关性,同时检测二者的表达,有助于宫颈癌的早期诊断。
OBJECTIVE: To investigate the expression of hTERT and p33ING1 gene in human uterine cervical squamous cell carcinoma of the Uygur women and the relationship between the expression of p33ING1 and telomerase reverse transcriptase catalytic subunit (hTERT). Methods: The expression of p33ING1 and hTERT in 52 cases of invasive cervical squamous cell carcinoma and 35 cases of cervical intraepithelial neoplasia were detected by immunohistochemical Envision method and compared with 13 cases of normal cervix. Results: ① The positive rates of p33ING1 in control group, CINII, I group, CINIII group and SCC group were 84.6%, 90%, 64%, 19.2% respectively. The expression of p33ING1 in SCC group was significantly lower than that in the control group (P <0.05). ② The positive rates of hTERT in the four groups were 15.4%, 70%, 96% and 100% respectively. The expression of hTERT in the cervical cancer tissues was significantly increased <0.05). ③ The expression of p33ING1 and hTERT was negatively correlated (p <0.05), but their expression levels were not significantly different from that of cancer tissue. Conclusion: The expression of p33ING1 in cervical cancer tissues of Uygur women in Xinjiang decreased significantly, while the expression of hTERT was significantly increased. The correlation between the expression of p33ING1 and the expression of hTERT in cervical cancer was significant. It is helpful for the early diagnosis of cervical cancer.