以苯肾上腺素为激动剂,在无钙K-H液中诱发大鼠去内皮主动脉环收缩并随后复钙的方法,观察粉防已碱和甲基莲心碱对主动脉环Ⅰ相收缩(胞内Ca~(++)释放)和Ⅱ相收缩(胞外Ca~(++)内流)的效应。结果表明,2×10~(-5)mol/L粉防己碱或甲基莲心碱预孵20min后,对Ⅰ、Ⅱ相的收缩均有非常明显的抑制作用(抑制率>92%,P<0.001);而短时预孵(5～10min)两药仅对Ⅲ相收缩有抑制作用,甲基莲心碱比粉防己碱起效慢、作用弱。 To phenylephrine as agonist in the absence of calcium-KH solution induced by rat aortic ring contraction and then recalcification of calcium method to observe the powder and alkali and neferine on the aortic ring Ⅰ phase contraction (intracellular Ca ~ (++) release) and Ⅱ phase contraction (extracellular Ca ~ (++) influx) effect. The results showed that 2 × 10 ~ (-5) mol / L tetrandrine or neferine preincubated for 20min, the Ⅰ, Ⅱ phase contraction have a very significant inhibition (inhibition rate> 92%, P < 0.001). Short-term preincubation (5 ~ 10 min) inhibited the contraction of phase Ⅲ only. The effect of neferine was slower and less effective than tetrandrine.